What question did this study set out to answer?

To purify and characterize the 40-kDa colon autoantigen (P40) targeted by colon-eluted antibodies in ulcerative colitis.

March 1, 1993The Journal of ImmunologyOpen Access

Autoimmunity to cytoskeletal protein tropomyosin. A clue to the pathogenetic mechanism for ulcerative colitis

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Authors

KDK M DasJohnson University ADA DasguptaChittaranjan National Cancer Institute AMAditya MandalUniversity of Calcutta

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Implication

Laboratory investigation uncovers autoimmune antibodies targeting cytoskeletal tropomyosin in ulcerative colitis, suggesting a direct pathogenetic mechanism.

Key Points

To purify and characterize the 40-kDa colon autoantigen (P40) targeted by colon-eluted antibodies in ulcerative colitis.
Purified the 40-kDa protein (P40) from human colon tissue extract to apparent homogeneity.
Performed proteolytic digestion and sequenced two internal peptides to classify the protein.
Screened blood serum from patients with ulcerative colitis for antibody reactivity against tropomyosin.
Peptide sequence analysis established that the colon autoantigen P40 belongs to the cytoskeletal tropomyosin family.
Circulating antibodies directed against tropomyosin were detected in 95% of patients with ulcerative colitis.

Cite This Study

Das et al. (1993) studied this question.

synapsesocial.com/papers/6a75570cf2cd31a1fadc063e https://doi.org/10.4049/jimmunol.150.6.2487

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Implication

Laboratory investigation uncovers autoimmune antibodies targeting cytoskeletal tropomyosin in ulcerative colitis, suggesting a direct pathogenetic mechanism.

Autoimmunity to cytoskeletal protein tropomyosin. A clue to the pathogenetic mechanism for ulcerative colitis

Discussion

Member takes

Implication

Key Points

Cite This Study

Autoimmunity to cytoskeletal protein tropomyosin. A clue to the pathogenetic mechanism for ulcerative colitis

Discussion

Member takes

Implication

Key Points

Cite This Study

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