What question did this study set out to answer?

This research aims to investigate how glutamatergic mechanisms in the retrotrapezoid nucleus affect sympathetic nerve discharge and respiratory responses to chemoreceptor activation.

July 9, 2011Experimental PhysiologyOpen Access

Contribution of excitatory amino acid receptors of the retrotrapezoid nucleus to the sympathetic chemoreflex in rats

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Population

Urethane-anaesthetized, vagotomized, sino-aortic denervated and artificially ventilated male Wistar rats

Comparison

Bilateral injection of NMDA receptor antagonist… vs Responses to hypercapnia or intravenous NaCN…

Design

Preclinical

Authors

ATAna C. TakakuraUniversidade de São Paulo TMThiago S. MoreiraUniversidade de São Paulo

Discussion

Member takes

Implication

Implicates RTN NMDA receptors in rat chemoreflex sympathoexcitation; leaves open translation to human cardiovascular control.

Key Points

This research aims to investigate how glutamatergic mechanisms in the retrotrapezoid nucleus affect sympathetic nerve discharge and respiratory responses to chemoreceptor activation.
Evaluated changes in splanchnic sympathetic nerve discharge (sSND) and phrenic nerve discharge (PND) in male Wistar rats during hypercapnia.
Administered NMDA and non-NMDA receptor antagonists in the retrotrapezoid nucleus and recorded physiological responses.
Used urethane anesthesia, vagotomy, and sino-aortic denervation to facilitate measurements of arterial pressure and nerve discharge.
Hypercapnia increased mean arterial pressure (MAP) by 32 ± 4 mmHg, sSND by 104 ± 4%, and PND amplitude by 101 ± 5%.
Bilateral injection of NMDA antagonist AP-5 reduced MAP, sSND, and PND responses significantly during hypercapnia (P<0.05).
Injection of DNQX reduced phrenic activation, while MCPG had no effect on responses in the retrotrapezoid nucleus.

Structured PICO

Population

Urethane-anaesthetized, vagotomized, sino-aortic denervated and artificially ventilated male Wistar rats

Intervention

Bilateral injection of NMDA receptor antagonist AP-5 (100 mm in 50 nl), non-NMDA receptor antagonist DNQX (100 mm in 50 nl), or metabotropic receptor antagonist MCPG (100 mm in 50 nl) in the retrotrapezoid nucleus (RTN)

Comparator

Responses to hypercapnia (10% CO2) or intravenous NaCN (50 μg kg-1) prior to antagonist injection

Outcome

Changes in mean arterial pressure (MAP), splanchnic sympathetic nerve discharge (sSND), and phrenic nerve discharge (PND)surrogate

RTN ionotropic glutamatergic receptors are involved in the sympathetic and respiratory responses produced by central and peripheral chemoreceptor activation in rats.

Cite This Study

Takakura et al. (2011) studied this question.

synapsesocial.com/papers/6a75599ff1e99ff85ab1e379 https://doi.org/10.1113/expphysiol.2011.058842

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Contribution of excitatory amino acid receptors of the retrotrapezoid nucleus to the sympathetic chemoreflex in rats

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Contribution of excitatory amino acid receptors of the retrotrapezoid nucleus to the sympathetic chemoreflex in rats

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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