Comparison of coagulation factor Xa and des-(1-44)factor Xa in the assembly of prothrombinase.
Population
In vitro biochemical system using factor Xa, Gla-domainless factor Xa, factor Va, prothrombin, and…
Comparison
Gla-domainless factor Xa vs Intact factor Xa (with and without phospholipid)
Design
Preclinical
Authors
Discussion
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Implication
Highlights Gla-domain dependence for phospholipid effects in prothrombinase; leaves open clinical translation to anticoagulation strategies.
Structured PICO
P
Population
In vitro biochemical system using factor Xa, Gla-domainless factor Xa, factor Va, prothrombin, and phospholipid
I
Intervention
Gla-domainless factor Xa (with and without phospholipid)
C
Comparator
Intact factor Xa (with and without phospholipid)
O
Outcome
Apparent dissociation constants (Kd') for the interaction of factor Va with factor Xa or Gla-domainless factor Xasurrogate
This biochemical study demonstrates that phospholipid has little or no effect on factor Va function when factor Xa has lost its Gla-mediated Ca2+-binding sites.
Cite This Study
Skogen et al. (1984) studied this question.