Why the study?
Salt-sensitive hypertension is common in China, but few studies have focused directly on patients with the condition.
What are the differentially expressed genes and pathways associated with salt-sensitive hypertension compared to salt-resistant hypertension?
What are the differentially expressed genes and pathways associated with salt-sensitive hypertension compared to salt-resistant hypertension?
Transcriptomic profiling of PBMCs in salt-sensitive hypertension identified key immune-related pathways and hub genes that may serve as potential biomarkers or therapeutic targets.
Answers are grounded in this paper and cite their sources.
Yields candidate SSH biomarkers from small-cohort PBMC RNA-seq; hypothesis-generating and requires validation before any clinical translation.
Background: Salt-sensitive hypertension (SSH) is a common type of essential hypertension in China. In recent years, although an increasing number of researches have focused on SSH, few studies have been researched on patients with SSH. The objective of this study was to explore the genes and pathways linked with SSH using RNA-sequencing (RNA-seq). Materials and methods: We used RNA-seq to analyze the transcriptome of peripheral blood mononuclear cells (PBMCs) of five SSH patients and five SRH patients. Next, we analyzed the differentially expressed genes (DEGs) using Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway, and Gene Set Enrichment (GSEA) enrichment analysis. Then, Cytoscape was used to construct the protein-protein interaction (PPI) network and the hub genes. Finally, CMAP analysis found that several small molecular compounds could reverse the altered DEGs. Results: A total of 431 DEGs were found in the PBMC samples, including 294 up-regulated and 137 down-regulated genes. Functional enrichment analysis found significant enrichment in immune-related associations such as inflammation, chemokine, and cytokine-cytokine receptor interaction. The hub genes of the two modules were IL-6, IL-1A, CCL2, CCL3L3, and BUB1. In addition, we identified two small molecular compounds (iopromide and iloprost) that potentially interacted with DEGs. Conclusion: This study suggests some potential biomarkers for the diagnosis of SSH. It provides new insights into SSH diagnosis and possible future clinical treatment.
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Chao et al. (2022) studied this question.
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