Direct conversion of lipid components to their fatty acid methyl esters

Summary Methyl esters were prepared from cholesteryl esters, phospholipids, and glycerides in substantially quantitative yields by methanolysis with large excess of sodium or potassium methoxide in absolute methanol. A silicic acid chromatographic adsorption column technique was described, which was effective in separating methyl esters from unsaponifiables such as sterols, pigments, etc., and free acids. Conditions for complete methanolysis of glyceride fats and oils requiring only 5 min. of reflux time were described. Quantitative conversion of fatty acids to methyl esters was accomplished by direct esterification with absolute methanol containing 4% HCL or H 2 SO 4 and by methylation with diazomethane.