Myo3 is a second type-II myosin heavy chain in S. pombe that cooperates with Myo2 in F-actin ring formation and cytokinesis.
Hypothesis-generating for myosin cooperation in cytokinesis; extends yeast models but leaves open relevance to mammalian cardiac cell biology.
We cloned the myo3+ gene of Schizosaccharomyces pombe which encodes a type-II myosin heavy chain. myo3 null cells showed a defect in cytokinesis under certain conditions. Overproduction of Myo3 also showed a defect in cytokinesis. Double mutant analysis indicated that Myo3 genetically interacts with Cdc8 tropomyosin and actin. Myo3 may be implicated in cytokinesis and stabilization of F-actin cables. Moreover, the function of Myo2 can be replaced by overexpressed Myo3. We observed a modest synthetic interaction between Myo2 and Myo3. Thus, Myo2 and Myo3 seem to cooperate in the formation of the F-actin ring in S. pombe.
No takes yet. Share an insight, caveat, or question.
Motegi et al. (1997) studied this question.
Synapse has enriched 4 closely related papers on similar clinical questions. Consider them for comparative context: