What question did this study set out to answer?

To investigate the causal role and biological effects of activating ras mutations in thyroid cell transformation and tumor development.

January 1, 1992Molecular Carcinogenesis

Stepwise transformation of primary thyroid epithelial cells by a mutant Ha‐ras oncogene: An in vitro model of tumor progression

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Authors

JBJorge S. BurnsUniversity of Ferrara JBJeremy P. BlaydesUniversity of Southampton PWPat A. WrightUniversity of Leeds

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Implication

In vitro experimental study demonstrates stepwise malignant transformation of rat thyroid epithelial cells via mutant c-Ha-ras, indicating an initiating role in benign and malignant tumor progression.

Key Points

To investigate the causal role and biological effects of activating ras mutations in thyroid cell transformation and tumor development.
Introduced a mutant c-Ha-ras oncogene into primary rat thyroid follicular cells using an ecotropic retroviral vector.
Maintained cell cultures continuously to assess proliferative lifespan, differentiation status, growth-factor dependence, and spontaneous malignant transformation.
Mutant c-Ha-ras expression expanded the cellular lifespan from less than 3 to more than 15 doublings without altering growth-factor dependence or differentiated traits.
Continuous culture resulted in rare spontaneous transformation into immortal, growth-factor-independent, and highly tumorigenic cell lines.
Malignant transformation was linked to the loss of growth inhibition by TGF-beta 1 and elevated nuclear p53 levels occurring without mutations in conserved coding regions.

Cite This Study

Burns et al. (1992) studied this question.

synapsesocial.com/papers/6a75866c9384a185e25ffacc https://doi.org/10.1002/mc.2940060208

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Stepwise transformation of primary thyroid epithelial cells by a mutant Ha‐ras oncogene: An in vitro model of tumor progression

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Implication

Key Points

Cite This Study

Stepwise transformation of primary thyroid epithelial cells by a mutant Ha‐ras oncogene: An in vitro model of tumor progression

Discussion

Member takes

Implication

Key Points

Cite This Study

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