Hens fed on a diet containing 0–05% calcium virtually ceased egg laying (production <4%) whereas those fed on a diet containing 0–5% Ca maintained production at between 20 and 30%. 2. After a return to a normal diet (3% Ca) egg weight, shell weight, ovarian characteristics and oviduct size were of a similar nature in the two groups, though the differences in egg weight, shell weight and shell calcium were significant at the 1 % level. 3. Bone weight and bone ash did not differ significantly between the groups but after a return to normal rations, bone weight and bone ash became higher in the group that had received the 0.05% Ca diet. 4. There were no clinical signs of bone demineralisation during the experiments.
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Gilbert et al. (1975) studied this question.
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