What question did this study set out to answer?

To determine whether antimicrobial imidazoles coordinate microbial flavohemoglobin and inhibit its nitric oxide dioxygenase activity.

April 26, 2005Antimicrobial Agents and ChemotherapyOpen Access

Imidazole Antibiotics Inhibit the Nitric Oxide Dioxygenase Function of Microbial Flavohemoglobin

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Authors

RHRyan A. HelmickMethodist University Hospital AFArin E. FletcherCincinnati Children's Hospital Medical Center AGAnne M. GardnerCornell University

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Implication

Experimental study demonstrates that imidazole antibiotics inhibit nitric oxide dioxygenase in bacteria and fungi, suggesting a novel target for antimicrobial drug engineering.

Key Points

To determine whether antimicrobial imidazoles coordinate microbial flavohemoglobin and inhibit its nitric oxide dioxygenase activity.
Measured the inhibition of Escherichia coli flavohemoglobin nitric oxide dioxygenase activity by miconazole, econazole, clotrimazole, and ketoconazole.
Evaluated inhibitory sensitivity across Saccharomyces cerevisiae, Candida albicans, and Alcaligenes eutrophus enzymes and assessed heme iron coordination and reduction.
Miconazole, econazole, clotrimazole, and ketoconazole inhibited E. coli flavohemoglobin with apparent Ki values of 80 nM, 550 nM, 1,300 nM, and 5,000 nM, respectively.
Imidazoles bound the heme iron atom, impaired ferric heme reduction, caused uncompetitive inhibition with respect to oxygen and nitric oxide, and inhibited microbial nitric oxide metabolism.

Cite This Study

Helmick et al. (2005) studied this question.

synapsesocial.com/papers/6a75897dc96e4a414b5ffce2 https://doi.org/10.1128/aac.49.5.1837-1843.2005

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Implication

Experimental study demonstrates that imidazole antibiotics inhibit nitric oxide dioxygenase in bacteria and fungi, suggesting a novel target for antimicrobial drug engineering.

Imidazole Antibiotics Inhibit the Nitric Oxide Dioxygenase Function of Microbial Flavohemoglobin

Discussion

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Implication

Key Points

Cite This Study

Imidazole Antibiotics Inhibit the Nitric Oxide Dioxygenase Function of Microbial Flavohemoglobin

Discussion

Member takes

Implication

Key Points

Cite This Study

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