What question did this study set out to answer?

This study aims to identify a mitochondria-active phenotype of hepatocellular carcinoma (HCC) using hyperpolarized pyruvate NMR spectroscopy and evaluate its sensitivity to mitochondrial inhibitors.

August 7, 2026NMR in Biomedicine

Hyperpolarized [2– ¹³ C]Pyruvate Identifies a Mitochondria‐Active Hepatocellular Carcinoma Phenotype With Vulnerability to Mitochondrial Inhibition

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This study aims to identify a mitochondria-active phenotype of hepatocellular carcinoma (HCC) using hyperpolarized pyruvate NMR spectroscopy and evaluate its sensitivity to mitochondrial inhibitors.
Used hyperpolarized [2–13 C]pyruvate NMR spectroscopy for metabolic profiling of McA-RH7777 HCC cells and N1S1 reference cells.
Assessed cell viability after treatment with glutaminase inhibitor BPTES and mitochondrial inhibitor CPI-613 using MTT assay.
Evaluated metabolic changes following CPI-613 treatment using hyperpolarized pyruvate spectroscopy.
HP [2–13 C]pyruvate enabled enhanced pyruvate-to-glutamate conversion in McA-RH7777 cells compared to N1S1.
CPI-613 treatment reduced cell viability in a dose-dependent manner; BPTES had limited effects.
Metabolic adaptation observed with increased pyruvate-to-lactate conversion following CPI-613 treatment.

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Implication

Randomized trial investigates mitochondrial activity in hepatocellular carcinoma, suggesting new avenues for targeted treatments.

Cite This Study

Dou et al. (2026) studied this question.

synapsesocial.com/papers/6a758bae847ab6d26c01f11b https://doi.org/10.1002/nbm.70360

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Hyperpolarized [2– ¹³ C]Pyruvate Identifies a Mitochondria‐Active Hepatocellular Carcinoma Phenotype With Vulnerability to Mitochondrial Inhibition

Key Points

Discussion

Implication

Cite This Study

Hyperpolarized [2– ¹³ C]Pyruvate Identifies a Mitochondria‐Active Hepatocellular Carcinoma Phenotype With Vulnerability to Mitochondrial Inhibition

Key Points

Discussion

Implication

Cite This Study

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