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August 7, 2026Non-Coding RNAOpen Access

Fraction-Seq: An Integrated Experimental and Computational Workflow for Determining the Localization and Abundance of Small Non-Coding RNAs in Subcellular Compartments

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Authors

SSSiddhartha ShahTCTess CherlinYJYi Jing

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Overview

Randomized trial determines subcellular localization and abundance of small non-coding RNAs, suggesting refined characterization methods.

Key Points

  • To develop a method for accurately determining the localization and abundance of small non-coding RNAs (sncRNAs) in subcellular compartments.
  • Developed an integrated workflow named 'Fraction-seq' combining experimental and analytical components.
  • Removed cross-fraction contamination to reconstruct true abundance of sncRNAs across fractions.
  • Applied across various adherent cells from any organism using R programming language for analysis.
  • Fraction-seq enables accurate reconstruction of abundance for all categories of sncRNAs.
  • Observed complex subcellular localization patterns that vary by sncRNA sequence and cell type.
  • The approach is publicly available via GitHub for further use and validation.

Cite This Study

Shah et al. (2026) studied this question.

synapsesocial.com/papers/6a758bc2847ab6d26c01f359https://doi.org/10.3390/ncrna12040029
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