What question did this study set out to answer?

This research aims to develop a streamlined method for quantifying low-abundance vitamin D3 metabolites in plasma.

August 7, 2026Rapid Communications in Mass Spectrometry

Systematic Optimization of an SLE‐DAPTAD LC–MS/MS Workflow for Simultaneous Quantification of Low‐Abundance Vitamin D ₃ Metabolites in Plasma

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This research aims to develop a streamlined method for quantifying low-abundance vitamin D3 metabolites in plasma.
Five sample-preparation approaches evaluated: protein precipitation, liquid–liquid extraction, salting-out, solid-phase extraction, supported liquid extraction.
Two derivatization reagents compared: DAPTAD and FMP-TS.
Method validated according to bioanalytical criteria.
Supported liquid extraction using ethyl acetate/methyl tert-butyl ether showed the best balance of signal intensity and reproducibility.
DAPTAD derivatization provided a significantly higher MS response compared to FMP-TS.
Method showed good linearity (r2 > 0.99), accuracy and precision, recovery rates between 72.5% and 104%, and limits of quantification of 0.08 ng/mL for specific metabolites.

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Implication

Randomized trial compares extraction and derivatization strategies for quantifying vitamin D metabolites in plasma, indicating a simplified workflow for clinical use.

Cite This Study

Amirmostofian et al. (2026) studied this question.

synapsesocial.com/papers/6a758c1b847ab6d26c01ff29 https://doi.org/10.1002/rcm.70158

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Implication

Randomized trial compares extraction and derivatization strategies for quantifying vitamin D metabolites in plasma, indicating a simplified workflow for clinical use.

Systematic Optimization of an SLE‐DAPTAD LC–MS/MS Workflow for Simultaneous Quantification of Low‐Abundance Vitamin D ₃ Metabolites in Plasma

Key Points

Discussion

Implication

Cite This Study

Systematic Optimization of an SLE‐DAPTAD LC–MS/MS Workflow for Simultaneous Quantification of Low‐Abundance Vitamin D ₃ Metabolites in Plasma

Key Points

Discussion

Implication

Cite This Study

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