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Investigate how glucagon, epinephrine, insulin, and calcium regulate intracellular cyclic AMP levels and gluconeogenesis in isolated hepatocytes from fed rats.

August 1, 1975Journal of Biological ChemistryOpen Access

Hormonal control of cyclic 3':5'-AMP levels and gluconeogenesis in isolated hepatocytes from fed rats

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Investigate how glucagon, epinephrine, insulin, and calcium regulate intracellular cyclic AMP levels and gluconeogenesis in isolated hepatocytes from fed rats.
Treated isolated rat hepatocytes with varying concentrations of glucagon, epinephrine, exogenous cyclic AMP, and insulin in the presence or absence of theophylline and probenecid.
Assayed gluconeogenesis from 2 mM lactate and monitored intracellular and extracellular cyclic AMP concentrations over time.
Evaluated the impact of calcium deficiency and measured 45Ca2+ influx into liver tissue following glucagon stimulation.
Glucagon and exogenous cyclic AMP stimulated gluconeogenesis nearly 4-fold (half-maximal at 0.3 nM glucagon and 30 µM cyclic AMP), whereas epinephrine stimulated it 1.5-fold (half-maximal at 0.2 µM).
Glucagon increased intracellular cyclic AMP up to 30-fold, while 10 nM insulin reduced glucagon-induced cyclic AMP accumulation and gluconeogenesis by approximately 50% without altering basal cyclic AMP or inhibiting adenylate cyclase.
Calcium deficiency reduced glucagon-stimulated gluconeogenesis by 50% without impairing cyclic AMP accumulation, while glucagon rapidly enhanced 45Ca2+ flux into the hepatocytes.

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Implication

Laboratory experiment reveals hormonal regulation of cyclic AMP and gluconeogenesis in rat liver cells, indicating calcium and insulin modulate glucagon signaling.

Cite This Study

Pilkis et al. (1975) studied this question.

synapsesocial.com/papers/6a758f9df0e270a4f3b1ca0c https://doi.org/10.1016/s0021-9258(19)41070-3

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Implication

Laboratory experiment reveals hormonal regulation of cyclic AMP and gluconeogenesis in rat liver cells, indicating calcium and insulin modulate glucagon signaling.

Hormonal control of cyclic 3':5'-AMP levels and gluconeogenesis in isolated hepatocytes from fed rats

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Discussion

Implication

Cite This Study

Hormonal control of cyclic 3':5'-AMP levels and gluconeogenesis in isolated hepatocytes from fed rats

Key Points

Discussion

Implication

Cite This Study