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March 1, 1999AJP Heart and Circulatory Physiology

Flow regulation of ecNOS and Cu/Zn SOD mRNA expression in porcine coronary arterioles

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Why the study?

Does high intraluminal flow increase ecNOS and Cu/Zn SOD mRNA expression in porcine coronary arterioles?

Population

Single porcine coronary arterioles (ID 100-160 micrometers; pressurized)

Comparison

High intraluminal flow for 2 or 4 hours vs Low flow or no flow for 2 or 4 hours

Design

Preclinical

Follow-up

2 or 4 hours

Authors

CWChristopher R. WoodmanTexas A&M UniversityJMJudy M. Muller‐DelpFlorida State UniversityJRJames W. E. RushPurchase College

Discussion

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Implication

High-flow upregulation of ecNOS/SOD in isolated porcine arterioles warrants no clinical change; leaves open shear-dependent endothelial adaptation in human coronary disease.

Key Points

  • To test whether increased intraluminal flow and shear stress in isolated coronary arterioles upregulate ecNOS and Cu/Zn SOD mRNA expression.
  • Cannulated and pressurized isolated single porcine coronary arterioles (internal diameter 100–160 µm) exposed to high flow (7.52 ± 0.22 µl/min; shear stress 5.7 ± 1.0 dyn/cm²), low flow (0.84 ± 0.05 µl/min; shear stress 1.6 ± 1.0 dyn/cm²), or no flow for 2 or 4 hours.
  • Extracted mRNA from perfused vessels and assessed ecNOS and Cu/Zn SOD gene expression using semiquantitative RT-PCR normalized to GAPDH.
  • ecNOS mRNA expression increased after both 2 hours and 4 hours of exposure to high flow.
  • Cu/Zn SOD mRNA expression increased only after 4 hours of high flow.
  • Neither ecNOS nor Cu/Zn SOD gene expression was induced by low flow at either timepoint.

Structured PICO

Does high intraluminal flow increase ecNOS and Cu/Zn SOD mRNA expression in porcine coronary arterioles?

P
Population
Single porcine coronary arterioles (ID 100-160 micrometers; pressurized)
I
Intervention
High intraluminal flow (7.52 +/- 0.22 microliter/min, mean shear stress 5.7 +/- 1.0 dyn/cm2) for 2 or 4 hours
C
Comparator
Low flow (0.84 +/- 0.05 microliter/min, mean shear stress 1.6 +/- 1.0 dyn/cm2) or no flow for 2 or 4 hours
O
Outcome
ecNOS and Cu/Zn SOD mRNA expression assessed using semiquantitative RT-PCRsurrogate

A threshold level of flow and shear stress must be sustained to elicit the upregulation of ecNOS and SOD mRNA expression in porcine coronary arterioles.

Cite This Study

Woodman et al. (1999) studied this question.

synapsesocial.com/papers/6a75978d20ccc22d0a67a66ahttps://doi.org/10.1152/ajpheart.1999.276.3.h1058
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Modulation of endothelium-dependent responses by chronic alterations of blood flow1986 · 134 citations
  2. 2Microvascular distribution of coronary vascular resistance in beating left ventricle1986 · 379 citations
  3. 3Shear Stress Modulates Expression of Cu/Zn Superoxide Dismutase in Human Aortic Endothelial Cells1996 · 299 citations
  4. 4Regulation of endothelial cell nitric oxide synthase mRNA expression by shear stress1995 · 569 citations
  5. 5Shear stress induction of the endothelial nitric oxide synthase gene is calcium-dependent but not calcium-activated1997 · 113 citations