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May 1, 1997Journal of Cellular Physiology

Shear stress induction of the endothelial nitric oxide synthase gene is calcium-dependent but not calcium-activated

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Population

Cultured bovine aortic endothelial cells (BAEC)

Comparison

Shear stress exposure for 6 hours, with or… vs Baseline/unstimulated cells

Design

Preclinical

Key result

Shear stress exposure of 4 and 25 dynes/cm2 increased the eNOS/GAPDH mRNA ratio in bovine aortic endothelial cells by 4.8- and 7.95-fold, respectively, in a calcium-dependent manner.

Authors

ZXZeshuai XiaoZZZhaohui ZhangSDScott L. Diamond

Discussion

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Overview

Hypothesis-generating for calcium-permissive eNOS regulation by shear; should not yet change clinical practice.

Structured PICO

P
Population
Cultured bovine aortic endothelial cells (BAEC)
I
Intervention
Shear stress exposure (4 and 25 dynes/cm2) for 6 hours, with or without pharmacological agents (dexamethasone, BAPTA/AM, ionomycin, genistein, tyrphostin B46, GDP-betaS)
C
Comparator
Baseline/unstimulated cells
O
Outcome
eNOS/GAPDH messenger RNA (mRNA) ratiosurrogate

Main Result

Effect estimate: 4.8- and 7.95-fold increase

Shear stress induction of eNOS mRNA in endothelial cells is a calcium-dependent but not calcium-activated process.

Cite This Study

Xiao et al. (1997) studied this question. Shear stress was evaluated on eNOS/GAPDH mRNA ratio (4.8- and 7.95-fold increase). Shear stress exposure of 4 and 25 dynes/cm2 increased the eNOS/GAPDH mRNA ratio in bovine aortic endothelial cells by 4.8- and 7.95-fold, respectively, in a calcium-dependent manner.

synapsesocial.com/papers/6a2a173d9b8d83ce413f068dhttps://doi.org/10.1002/(sici)1097-4652(199705)171:2<205::aid-jcp11>3.0.co;2-c
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Molecular cloning and characterization of the constitutive bovine aortic endothelial cell nitric oxide synthase.1992 · 740 citations
  2. 2Structure and chromosomal localization of the human constitutive endothelial nitric oxide synthase gene1993 · 879 citations
  3. 3Tyrosine phosphorylation of paxillin and pp125FAK accompanies cell adhesion to extracellular matrix: a role in cytoskeletal assembly.1992 · 1,319 citations
  4. 4Shear Stress Modulates Expression of Cu/Zn Superoxide Dismutase in Human Aortic Endothelial Cells1996 · 297 citations
  5. 5Regulation of Endothelial Constitutive Nitric Oxide Synthase by Protein Kinase C1995 · 159 citations