Shear stress stimulates eNOS phosphorylation at Ser(635) via a PKA-dependent but PI3K-independent pathway, suggesting a role in chronic regulation of eNOS.
Shear stress stimulates eNOS phosphorylation at Ser(635) via a PKA-dependent but PI3K-independent pathway, suggesting a role in chronic regulation of eNOS.
Shear stress stimulates nitric oxide (NO) production by phosphorylating endothelial NO synthase (eNOS) at Ser(1179) in a phosphoinositide-3-kinase (PI3K)- and protein kinase A (PKA)-dependent manner. The eNOS has additional potential phosphorylation sites, including Ser(116), Thr(497), and Ser(635). Here, we studied these potential phosphorylation sites in response to shear, vascular endothelial growth factor (VEGF), and 8-bromocAMP (8-BRcAMP) in bovine aortic endothelial cells (BAEC). All three stimuli induced phosphorylation of eNOS at Ser(635), which was consistently slower than that at Ser(1179). Thr(497) was rapidly dephosphorylated by 8-BRcAMP but not by shear and VEGF. None of the stimuli phosphorylated Ser(116). Whereas shear-stimulated Ser(635) phosphorylation was not affected by phosphoinositide-3-kinase inhibitors wortmannin and LY-294002, it was blocked by either treating the cells with a PKA inhibitor H89 or infecting them with a recombinant adenovirus-expressing PKA inhibitor. These results suggest that shear stress stimulates eNOS by two different mechanisms: 1) PKA- and PI3K-dependent and 2) PKA-dependent but PI3K-independent pathways. Phosphorylation of Ser(635) may play an important role in chronic regulation of eNOS in response to mechanical and humoral stimuli.
Boo et al. (Fri,) reported a other. Shear stress, VEGF, and 8-BRcAMP was evaluated on Phosphorylation of eNOS at Ser(635), Thr(497), and Ser(116). Shear stress stimulates eNOS phosphorylation at Ser(635) via a PKA-dependent but PI3K-independent pathway, suggesting a role in chronic regulation of eNOS.
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