January 1, 2012Cellular Physiology and BiochemistryOpen Access

Downregulation of Kv1.5 K+Channels by the AMP-Activated Protein Kinase

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Population

Xenopus oocytes injected with cRNA encoding Kv1.5

Comparison

Co-injection of wild-type AMPK, constitutively… vs Xenopus oocytes injected with cRNA encoding…

Design

Preclinical

Authors

SMSobuj MiaUniversity of Rajshahi CMCarlos MuñozBoston Children's Hospital TPTatsiana PakladokUniversity of Tübingen

Discussion

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Implication

Hypothesis-generating for AMPK-Kv1.5 modulation in arrhythmias; should not yet change clinical practice.

Structured PICO

Population

Xenopus oocytes injected with cRNA encoding Kv1.5

Intervention

Co-injection of wild-type AMPK (α1 β 1γ1), constitutively active (γR70Q)AMPK (α1 β 1γ1(R70Q)), inactive mutant (αK45R)AMPK (α1(K45R)β1γ1), or Nedd4-2

Comparator

Xenopus oocytes injected with cRNA encoding Kv1.5 without additional injection

Outcome

Kv1.5 activity (determined by two-electrode voltage-clamp) and Kv1.5 protein abundance in the cell membranesurrogate

AMPK is a potent regulator of Kv1.5, inhibiting it presumably in part by activation of Nedd4-2 with subsequent clearance of channel protein from the cell membrane.

Cite This Study

Mia et al. (2012) studied this question.

synapsesocial.com/papers/6a7597bdfbccc8f4fd160fe5 https://doi.org/10.1159/000341480

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Downregulation of Kv1.5 K+Channels by the AMP-Activated Protein Kinase

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Downregulation of Kv1.5 K+Channels by the AMP-Activated Protein Kinase

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider