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September 3, 2022Open Access

Organoid-based single-cell spatiotemporal gene expression landscape of human embryonic development and hematopoiesis

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Authors

YCYiming ChaoChinese University of Hong Kong YXYang XiangBeijing University of Technology JXJiashun XiaoSun Yat-sen University

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Implication

Spatiotemporal transcriptomic study reveals embryonic tissue and blood cell differentiation in human organoids, highlighting key signaling pathways in development.

Key Points

To establish a stem-cell-derived organoid model that maps the spatiotemporal gene expression landscape of human embryonic development and hematopoiesis at single-cell resolution.
Generated human embryonic organoids (HEMO) from expanded potential stem cells, encompassing both embryonic and extraembryonic tissues.
Profiled cellular dynamics using time-series single-cell RNA sequencing (10x Chromium) integrated with single-cell resolution spatial transcriptomics (10x Visium).
Recapitulated development of extraembryonic placenta, cardiac mesoderm, neural crest, blood vessels, and predominant blood lineages including erythropoiesis, megakaryopoiesis, and myelopoiesis.
Identified that trophoblast-like tissues provide WNT signaling to drive neural crest maturation, while vitronectin-integrin signaling (VTN-ITGA2B) promotes megakaryocyte maturation within the yolk sac erythro-megakaryopoietic niche.

Cite This Study

Chao et al. (2022) studied this question.

synapsesocial.com/papers/6a759ff042ce5c1965dd8edd https://doi.org/10.1101/2022.09.02.505700

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Implication

Spatiotemporal transcriptomic study reveals embryonic tissue and blood cell differentiation in human organoids, highlighting key signaling pathways in development.

Organoid-based single-cell spatiotemporal gene expression landscape of human embryonic development and hematopoiesis

Discussion

Member takes

Implication

Key Points

Cite This Study

Organoid-based single-cell spatiotemporal gene expression landscape of human embryonic development and hematopoiesis

Discussion

Member takes

Implication

Key Points

Cite This Study

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