What question did this study set out to answer?

To investigate the prevalence and laboratory detection of newer beta-lactamases, including extended-spectrum beta-lactamases, imported AmpC, and carbapenemases, in Klebsiella pneumoniae isolates across U.S. hospitals.

November 15, 2002Antimicrobial Agents and ChemotherapyOpen Access

Occurrence of Newer β-Lactamases in Klebsiella pneumoniae Isolates from 24 U.S. Hospitals

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To investigate the prevalence and laboratory detection of newer beta-lactamases, including extended-spectrum beta-lactamases, imported AmpC, and carbapenemases, in Klebsiella pneumoniae isolates across U.S. hospitals.
Screened 1,123 K. pneumoniae isolates across 24 U.S. medical centers, including archived isolates from 1996 to 2000 showing intermediate or resistant profiles to cefoxitin or imipenem.
Characterized beta-lactamase production in 408 screen-positive isolates using phenotypic confirmatory tests, three-dimensional tests, isoelectric focusing, inhibitor assays, induction assays, spectrophotometric assays, and molecular tests.
Extended-spectrum beta-lactamase-producing isolates were confirmed at 18 of 24 sites (75%), and imported AmpC-producing isolates were detected at 10 sites (42%), with inducible imported AmpC present at 3 sites (12.5%).
A molecular class A carbapenem-hydrolyzing enzyme was identified at 1 site (4%), while no class B or class D carbapenemases were detected.

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Implication

Multicenter surveillance study reveals widespread dissemination of extended-spectrum and AmpC beta-lactamases in Klebsiella pneumoniae isolates, highlighting the need for improved laboratory...

Cite This Study

Moland et al. (2002) studied this question.

synapsesocial.com/papers/6a75a16312165deef3dd8985 https://doi.org/10.1128/aac.46.12.3837-3842.2002

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Implication

Multicenter surveillance study reveals widespread dissemination of extended-spectrum and AmpC beta-lactamases in Klebsiella pneumoniae isolates, highlighting the need for improved laboratory...

Occurrence of Newer β-Lactamases in Klebsiella pneumoniae Isolates from 24 U.S. Hospitals

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Discussion

Implication

Cite This Study

Occurrence of Newer β-Lactamases in Klebsiella pneumoniae Isolates from 24 U.S. Hospitals

Key Points

Discussion

Implication

Cite This Study

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