Key result
Periostin (POSTN) promotes keloid fibroblast migration and proliferation by activating the JAK/STAT signaling pathway.
Why the study?
Keloids exhibit abnormal proliferation of keloid fibroblasts, and this study investigated periostin and its mechanism to provide a reference for targeted keloid therapy.
Periostin promotes keloid fibroblast migration and proliferation via the JAK/STAT signaling pathway, highlighting a potential therapeutic target for keloid treatment.
No immediate clinical implications; leaves open whether JAK/STAT inhibition alters keloid outcomes.
A keloid is secondary to skin trauma or has spontaneously manifested as an overgrowth and occurs when the skin heals abnormally after an injury. The main pathological manifestations are abnormal proliferation of keloid fibroblasts (KEL-FIB). This study researched periostin (POSTN) on keloid fibroblasts (KEL-FIB) and the associated mechanism, aiming to provide a reference for the targeted therapy of keloid. We got tissues from Second People's Hospital of Guangxi Zhuang Autonomous Region between June 2022 and March 2023. POSTN expression was increased in keloid skin tissue and KEL-FIB than normal skin tissue and normal fibroblasts. We collected and inoculated KEL-FIB cells, transfection of si-NC (Silencing of POSTN negative control), si-POSTN (Silencing of POSTN), pcDNA-NC (Overexpression of POSTN negative control), and POSTN (Overexpression of POSTN) (Thermo Fisher Scientific) used Lipofectamine 2000 transfection reagent. Wound closure, cell proliferation viability, migrated cell numbers, and POSTN, p-JAK2, p-STAT3 protein levels were reduced in the si-POSTN group. Wound closure, cell proliferation viability, migrated cell numbers, and POSTN, p-JAK2, p-STAT3 protein levels were elevated in the POSTN group. POSTN protein levels did not changed and wound closure, cell proliferation viability, migrated cell numbers, were reduced in the POSTN + S-Ruxolitinib group. The study results indicated that POSTN promotes cell migration and proliferation by activating the JAK/STAT pathway, promoting KEL-FIB development.
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Tian et al. (2024) studied Keloid. POSTN silencing and overexpression vs. Negative controls was evaluated on Cell migration, proliferation, and JAK/STAT pathway activation. Periostin (POSTN) promotes keloid fibroblast migration and proliferation by activating the JAK/STAT signaling pathway.
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