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June 1, 1979Proceedings of the National Academy of SciencesOpen Access

Mechanism for discrimination between viral and host mRNA in interferon-treated cells.

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Population

HeLa S3 cells grown in suspension cultures

Comparison

Treatment with 100 NIH units of human fibroblast… vs Extracts from control (untreated) HeLa cells

Design

Preclinical

Authors

TNTimothy W. NilsenQuantuMDx (United Kingdom)CBCorrado BaglioniUniversity of Pisa

Discussion

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Implication

Supports dsRNA-triggered mRNA selectivity in interferon responses; leaves open in vivo validation and therapeutic translation.

Structured PICO

P
Population
HeLa S3 cells grown in suspension cultures
I
Intervention
Treatment with 100 NIH units of human fibroblast interferon per ml for 20 hours
C
Comparator
Extracts from control (untreated) HeLa cells
O
Outcome
Degradation of mRNA linked to double-stranded RNA (dsRNA) compared with mRNA not linked to dsRNAsurrogate

The study demonstrates that in interferon-treated cells, a localized activation of an endonuclease near dsRNA regions leads to preferential degradation of viral mRNA, providing a mechanism for discrimination between viral and host mRNA.

Cite This Study

Nilsen et al. (1979) studied this question.

synapsesocial.com/papers/6a75af81fbccc8f4fd16183fhttps://doi.org/10.1073/pnas.76.6.2600
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Also Consider

Synapse has enriched 3 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Synthesis of an Oligonucleotide Inhibitor of Protein Synthesis in Rabbit Reticulocyte Lysates Analogous to that Formed in Extracts from Interferon‐Treated Cells1978 · 165 citations
  2. 2Metabolic stability of 2′ 5′oligo (A) and activity of 2′ 5′oligo (A)-dependent endonuclease in extracts of interferon-treated and control HeLa cells1979 · 103 citations
  3. 3Partial reaction of peptide initiation inhibited by phosphorylation of either initiation factor eIF-2 or 40S ribosomal proteins.1977 · 61 citations