What question did this study set out to answer?

To identify and profile genes showing augmented expression levels in the peripheral blood cells of patients with systemic lupus erythematosus.

January 1, 2005DNA ResearchOpen Access

Isolation and Expression Profiling of Genes Upregulated in the Peripheral Blood Cells of Systemic Lupus Erythematosus Patients

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Authors

TITaeko IshiiTohoku Medical and Pharmaceutical University Hospital HOHiroaki OndaJackson Laboratory ATAkie TanigawaJuntendo University

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Implication

Case-control study reveals upregulation of interferon-inducible and novel genes in systemic lupus erythematosus, highlighting non-interferon pathways in disease pathophysiology.

Key Points

To identify and profile genes showing augmented expression levels in the peripheral blood cells of patients with systemic lupus erythematosus.
Screened blood cell gene expression using stepwise subtraction and microarray analysis.
Quantified and validated transcript levels via quantitative real-time RT-PCR in 31 patients with SLE and 30 healthy controls.
Compared expression profiles against patients with idiopathic thrombocytopenic purpura (ITP) and evaluated correlations with clinical laboratory measures.
Identified eight genes with significantly elevated transcription in SLE, including interferon-alpha-inducible genes (IFI27, G1P2, ISG20), EPSTI1, DEFA3, AREG, and two unknown genes (AL050290 and SLED1).
IFI27, G1P2, and SLED1 were preferentially upregulated in SLE compared to ITP, whereas AREG and AL050290 showed higher expression in ITP.
Transcription levels of ISG20, EPSTI1, and SLED1 correlated significantly with patient lymphocyte counts.

Cite This Study

Ishii et al. (2005) studied this question.

synapsesocial.com/papers/6a75bb8049a8cdd61fb1db6b https://doi.org/10.1093/dnares/dsi020

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Implication

Case-control study reveals upregulation of interferon-inducible and novel genes in systemic lupus erythematosus, highlighting non-interferon pathways in disease pathophysiology.

Isolation and Expression Profiling of Genes Upregulated in the Peripheral Blood Cells of Systemic Lupus Erythematosus Patients

Discussion

Member takes

Implication

Key Points

Cite This Study

Isolation and Expression Profiling of Genes Upregulated in the Peripheral Blood Cells of Systemic Lupus Erythematosus Patients

Discussion

Member takes

Implication

Key Points

Cite This Study

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