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Transfer RNA nucleotidyltransferase (EC 2.7.7.25) from Escherichia coli has been purified to near homogeneity.The apparent molecular weight of the enzyme is about 50,000 as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and gel filtration.No evidence for subunits was obtained.The enzyme is rapidly and irreversibly inactivated in the absence of magnesium.
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Miller et al. (1971) studied this question.
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