What question did this study set out to answer?

To systematically evaluate the membrane translocation efficiency, kinetics, stability, and cytotoxicity of oligo-arginines across different chain lengths, concentrations, and stereoisomers in living cells.

December 11, 2007Journal of Peptide Science

Live‐cell analysis of cell penetration ability and toxicity of oligo‐arginines

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Authors

GLGisela Lättig-TünnemannHumboldt-Universität zu Berlin GTGohar Ter‐AvetisyanHumboldt-Universität zu Berlin RMRobert M. MartinUniversity of Lisbon

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Implication

In vitro study demonstrates length- and isomer-dependent transduction and toxicity of oligo-arginines in live cells, highlighting optimal windows for peptide-based drug delivery.

Key Points

To systematically evaluate the membrane translocation efficiency, kinetics, stability, and cytotoxicity of oligo-arginines across different chain lengths, concentrations, and stereoisomers in living cells.
Analyzed live-cell membrane translocation of L-isoforms of oligo-arginines and lysines using confocal microscopy across varying chain lengths and concentrations.
Monitored real-time uptake kinetics and comparative transduction of L- and protease-resistant D-R9 stereoisomers across multiple cell types.
Assessed peptide-associated cytotoxicity and membrane disruption via propidium iodide staining, cell viability assays, and cell cycle progression analyses.
Arginine transduction increased with chain length and concentration, with L-R9 and L-R10 exhibiting the highest uptake, whereas oligo-lysines showed virtually no translocation.
D-R9 demonstrated consistently faster and higher transduction than L-R9 across all cell lines, with uptake differences varying widely among cell types.
Real-time dual-peptide tracking and cell cycle assays identified distinct concentration windows that achieve robust intracellular bioavailability with minimal cytotoxicity.

Cite This Study

Lättig-Tünnemann et al. (2007) studied this question.

synapsesocial.com/papers/6a75c8c342ce5c1965dda2bb https://doi.org/10.1002/psc.968

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Implication

In vitro study demonstrates length- and isomer-dependent transduction and toxicity of oligo-arginines in live cells, highlighting optimal windows for peptide-based drug delivery.

Live‐cell analysis of cell penetration ability and toxicity of oligo‐arginines

Discussion

Member takes

Implication

Key Points

Cite This Study

Live‐cell analysis of cell penetration ability and toxicity of oligo‐arginines

Discussion

Member takes

Implication

Key Points

Cite This Study

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