Green nanotechnology approaches using Mesobacillus jeotgali ADCG SIST 4 strain synthesized gold nanoparticles for anticancer studies

The current area of research explores the biosynthesis, characterization, and assessment of the cytotoxic actions of gold bio-nanoparticles produced by Mesobacillus jeotgali ADCG SIST 4. Mesobacillus jeotgali ADCG SIST 4, which is the least explored marine bacteria were utilized to study the eco-friendly production. This study is to determine whether the marine bacteria Mesobacillus jeotgali ADCG SIST 4 is capable of producing AuNPs extracellularly. AuNPs were characterized using a combination of spectroscopic and microscopic techniques. UV–vis absorbance spectra exhibited a surface plasmon resonance peak at 546 nm, indicating the presence of AuNPs. Additionally, the AuNPs displayed a mixture of hexagonal and spherical shapes, with particle sizes ranging from 2 to 80 nm. High-resolution transmission electron microscopy (HR-TEM) was employed to investigate and describe the shape and morphology of the synthesized AuNPs. The particle size analyzer and Zeta sizer revealed the average particle size of AuNPs as 78.6 nm and charge as −13.6 mv. XRD analysis revealed the crystalline nature of AuNPs. GC-MS analysis was performed to study the capping agents involved in AuNPs formation. On Vero and MCF-7 cell lines, a significant cytotoxic response of synthesized AuNPs was evaluated using the cytotoxicity assay . Apoptosis study of the synthesized AuNPs showed extensive toxicological effects on the MCF-7 cell line by assessing the morphology of cells using propidium iodide and acridine orange staining.