What question did this study set out to answer?

Determine whether epidermal growth factor receptor (EGFr) tyrosine kinase activity is necessary to trigger MAP kinase tyrosine phosphorylation, activation, and subsequent cellular mitogenesis.

July 1, 1992Journal of Biological ChemistryOpen Access

Tyrosine phosphorylation of mitogen-activated protein kinase in cells with tyrosine kinase-negative epidermal growth factor receptors.

View Full Paper

Ask AI

Bookmark

Determine whether epidermal growth factor receptor (EGFr) tyrosine kinase activity is necessary to trigger MAP kinase tyrosine phosphorylation, activation, and subsequent cellular mitogenesis.
Exposed cells expressing either normal human EGFr or mutant tyrosine kinase-inactive EGFr to epidermal growth factor (EGF).
Measured cellular protein tyrosine phosphorylation, MAP kinase phosphorylation and activation status, and EGF-dependent cellular proliferation.
EGF treatment of cells with tyrosine kinase-inactive EGFr failed to stimulate the tyrosine phosphorylation of endogenous cellular substrates.
Tyrosine phosphorylation and enzymatic activation of MAP kinase occurred despite the total absence of EGFr tyrosine kinase activity.
EGF failed to induce proliferation in cells carrying kinase-inactive EGFr, demonstrating that MAP kinase phosphorylation is insufficient by itself to stimulate mitogenesis.

Discussion

No takes yet. Share an insight, caveat, or question.

Implication

In vitro study reveals MAP kinase activation in kinase-inactive EGF receptor cells, indicating receptor kinase-independent signaling and showing MAP kinase alone cannot drive mitogenesis.

Cite This Study

Campos‐González et al. (1992) studied this question.

synapsesocial.com/papers/6a75d403ef3d015f2ddd8e7d https://doi.org/10.1016/s0021-9258(18)42070-4

View Full Paper

Ask AI

Bookmark

Implication

In vitro study reveals MAP kinase activation in kinase-inactive EGF receptor cells, indicating receptor kinase-independent signaling and showing MAP kinase alone cannot drive mitogenesis.

Tyrosine phosphorylation of mitogen-activated protein kinase in cells with tyrosine kinase-negative epidermal growth factor receptors.

Key Points

Discussion

Implication

Cite This Study

Tyrosine phosphorylation of mitogen-activated protein kinase in cells with tyrosine kinase-negative epidermal growth factor receptors.

Key Points

Discussion

Implication

Cite This Study

Keep reading

Keep reading