A comparison of currents carried by HERG, with and without coexpression of MiRP1, and the native rapid delayed rectifier current. Is MiRP1 the missing link?
Population
Chinese hamster ovary (CHO) cells and Xenopus oocytes expressing HERG, and guinea-pig cells with native I(Kr)
Comparison
Coexpression of MiRP1 with HERG vs HERG expression without MiRP1, and native I(Kr)
Design
Preclinical
Authors
Discussion
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Member takes
Implication
Challenges MiRP1 necessity for native I(Kr); hypothesis-generating for HERG channel composition in human cardiomyocytes.
Structured PICO
P
Population
Chinese hamster ovary (CHO) cells and Xenopus oocytes expressing HERG, and guinea-pig cells with native I(Kr)
I
Intervention
Coexpression of MiRP1 with HERG
C
Comparator
HERG expression without MiRP1, and native I(Kr)
O
Outcome
Pharmacological sensitivity (to quinidine, E-4031, dofetilide) and biophysical properties (deactivation, activation voltage dependence)surrogate
MiRP1 coexpression does not reduce the discrepancies between HERG currents and native I(Kr), suggesting it may not be an essential constituent of the native HERG channel complex.
Cite This Study
Weerapura et al. (2002) studied this question.