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Microbial methanogenesis was proved geochemically, based on the abundance of methanogenic bacteria and methane production rates in experiments with radioactive carbon. The results are compared with direct measurements of methane concentrations in mud samples taken with a hermetic sampler. The migration of methane formed in sediments occurs during filtration of porewater rather than at the expense of gas diffusion.
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Lein et al. (1981) studied this question.
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