Measurement of reference value range of serum inflammatory cytokines in normal population in Baoding area

Cytokines are a general term for polypeptide cell regulatory substances, including interleukin, interferons, growth factors, cell stimulating factors, tumor necrosis factor and so on. It is mainly composed of immune cells (T cells, B cells, macrophages, etc.) and some non-immune cells (endothelial cells, fibroblasts), a kind of small molecule proteins with a wide range of biological activity, with various functions such as regulating immunity, promoting blood cell generation, cell growth and repair of damaged tissues. Cytokines also play an important role in the pathogenesis, diagnosis, drug efficacy and prognosis evaluation of hematological and immune system diseases. However, in the clinical reports, the normal control has a small sample size, narrow coverage, regional differences and different detection techniques, and the reported results are lack of comparable, so it is very important to establish the reference value range of cytokines in the normal population in this region. At present, there is no epidemiological report of inflammatory cytokine levels in normal population in Baoding. Therefore, the flow cytometry microsphere array method (cytometric bead arry, CBA) can detect several soluble proteins at one time, The serum levels of IL-1 β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-8, IL-10, IL-12P70, IL-17A, IFN- α, TIFN- α and IFN- γ in multiple levels of cytokines, To provide a basis for determining the normal reference values of these factors in the Baoding area, It also lays the foundation for the subsequent research of related diseases.