Consumption of nutritionally enriched hen eggs enhances endothelium-dependent vasodilation via cyclooygenases’ metabolites in healthy young persons - A randomized study

Petar Šušnjara; Zrinka Mihaljević; Ana Stupin; Nikolina Kolobarić; Anita Matić; Ivana Jukić; Zlata Kralik; Gordana Kralik; Anđelina Miloloža; Tihana Pavošević; Vatroslav Šerić; Zdenko Lončarić; Darko Kerovec; Olivera Galović; Ines Drenjančević

doi:10.21203/rs.3.rs-2205230/v1

November 2, 2022Open Access

Consumption of nutritionally enriched hen eggs enhances endothelium-dependent vasodilation via cyclooygenases’ metabolites in healthy young persons - A randomized study

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Why the study?

The study aimed to evaluate the effects of consuming enriched hen eggs on endothelium-dependent vasodilation and the role of cyclooxygenases in microcirculation among young healthy individuals.

Does consumption of nutritionally enriched hen eggs improve endothelium-dependent vasodilation in healthy young persons?

Key Points

To assess the effects of consuming nutritionally enriched hen eggs on microvascular endothelium-dependent vasodilation and the contribution of the cyclooxygenase pathway in young healthy individuals.
In a randomized interventional trial (NCT04564690), healthy young participants consumed 3 eggs daily for 3 weeks: regular control eggs (n = 14) or enriched eggs containing elevated n-3 PUFAs, lutein, selenium, and vitamin E (n = 20).
Skin microvascular blood flow was measured using laser Doppler flowmetry during post-occlusive reactive hyperemia and iontophoresis of acetylcholine or sodium nitroprusside, both before and after oral administration of 100 mg indomethacin.
Hemodynamic markers, anthropometric metrics, lipid and liver panels, and protein expression levels of COX-1, COX-2, nNOS, iNOS, and eNOS were determined pre- and post-dietary protocol.
Post-occlusive reactive hyperemia and acetylcholine-induced vasodilation were significantly enhanced in the enriched egg group, whereas endothelium-independent vasodilation and all control group vascular measurements remained unchanged.
Administration of the nonselective cyclooxygenase inhibitor indomethacin significantly reduced post-occlusive reactive hyperemia exclusively in the enriched egg group after the intervention.
Microvascular protein expression of COX-2 was significantly higher in the enriched egg cohort compared to the control cohort following the 3-week diet.

Structured PICO

Does consumption of nutritionally enriched hen eggs improve endothelium-dependent vasodilation in healthy young persons?

P

Population

34 young healthy individuals (age 18-30 years, arterial BP ≤120/80 mmHg, BMI 18.5-24.9 kg/m2). Exclusion criteria: history of smoking, hyperlipidemia, renal impairment, hypertension, coronary artery disease, diabetes, cerebrovascular and peripheral artery disease, and taking any drugs or substances that could affect the endothelium, enriched functional food, or n-3 PUFAs, lutein, selenium, and vitamin E supplementation.

I

Intervention

3 enriched hen eggs per day for 3 weeks (providing approximately 1.85 mg lutein, 0.06 mg selenium, 3.29 mg vitamin E, and 1026 mg n-3 PUFAs daily).

C

Comparator

3 regular hen eggs per day for 3 weeks (providing approximately 0.330 mg lutein, 1.785 mg vitamin E, 0.054 mg selenium, and 438 mg n-3 PUFAs daily).

O

Outcome

Skin microvascular blood flow in response to endothelium-dependent vasodilation (post-occlusive reactive hyperemia [PORH] and iontophoresis of acetylcholine [AChID]) and endothelium-independent vasodilation (sodium nitroprusside [SNPID]) assessed by laser Doppler flowmetry before and after the 3-week dietary protocol.surrogate

Consumption of n-3 PUFA-enriched eggs for 3 weeks improves microvascular endothelium-dependent vasodilation in healthy young individuals, likely mediated via the cyclooxygenase pathway.

Implication

May improve microvascular EDV in healthy young adults; leaves open clinical relevance in at-risk populations.

Abstract

Background Present study aimed to evaluate the effects of enriched hen eggs consumption on the endothelium-dependent vasodilation (EDV) and the role of cyclooxygenases in EDV in microcirculation of young healthy individuals. Methods Participants consumed 3 eggs per day for 3 weeks: Control group (n = 14) consumed regular hen eggs (approximately 0.330 mg of lutein, 1.785 mg of vitamin E, 0.054 mg of selenium and 438 mg of n-3 PUFAs daily) and Nutri4 group (n = 20) consumed enriched eggs (approximately 1.85 mg of lutein, 0.06 mg of selenium, 3.29 mg of vitamin E, and 1026 mg of n-3 PUFAs daily). Skin microvascular blood flow in response to EDV (post-occlusive reactive hyperemia (PORH) and iontophoresis of acetylcholine (AChID)) and sodium nitroprusside (SNPID; endothelium-independent) were assessed by laser Doppler flowmetry before and after dietary protocol, with or without 100 mg of indomethacin perorally. Arterial blood pressure, heart rate, serum lipid and liver enzymes, anthropometric measurements, protein expression of COX-1, COX-2, nNOS, iNOS, and eNOS were measured before and after dietary protocol. Results PORH and AChID were significantly enhanced, and SNPID remained unchanged in Nutri4 group, while none was changed in Control group following respective diet. PORH was decreased after administration of indomethacin in Nutri4 after dietary protocol. Protein expression of COX-2 was significantly higher in the Nutri4 group compared to Control group after dietary protocol. Conclusion Consumption of enriched eggs improves microvascular EDV in healthy young subjects. Results suggest involvement of n-3 PUFAs metabolites via the cyclooxygenases pathway in enhanced reactive hyperemia. Trial registration : The study was registered on the Clinical trial under the title: Effect of Enriched QUARTET® Hen Eggs on Cardiovascular Function in Cardiovascular Patients and Healthy Individuals. NCT number: NCT04564690 on September 2020 (https://clinicaltrials.gov/ct2/show/NCT04564690).

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Cite This Study

Šušnjara et al. (2022) studied this question.

synapsesocial.com/papers/6a75f3f249d3dc94b0786617 https://doi.org/10.21203/rs.3.rs-2205230/v1

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Consumption of nutritionally enriched hen eggs enhances endothelium-dependent vasodilation via cyclooygenases’ metabolites in healthy young persons - A randomized study

Key Points

Structured PICO

Implication

Abstract

Discussion

Cite This Study

Consumption of nutritionally enriched hen eggs enhances endothelium-dependent vasodilation via cyclooygenases’ metabolites in healthy young persons - A randomized study

Key Points

Structured PICO

Implication

Abstract

Discussion

Cite This Study

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