What question did this study set out to answer?

To develop and validate a precise, cost-effective, and environmentally sustainable RP-HPLC method for the simultaneous quantification of dapagliflozin, linagliptin, and metformin.

September 28, 2025INDIAN DRUGS

Development and Validation of Rp-HPLC for Simultaneous Quantitation of Linagliptin, Dapagliflozin Propanediol Monohydrate and Metformin Hydrochloride: Greenness Evaluation by Agree and Gapi Tools

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Authors

SRSapna M. Rathod SPSayema Pothigara NPNisarg C. Patel

Discussion

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Overview

Method validation study demonstrates precise simultaneous RP-HPLC quantitation of linagliptin, dapagliflozin, and metformin, indicating its utility for eco-friendly pharmaceutical quality control.

Key Points

To develop and validate a precise, cost-effective, and environmentally sustainable RP-HPLC method for the simultaneous quantification of dapagliflozin, linagliptin, and metformin.
Chromatographic separation was achieved on an Agilent Eclipse XBD C18 column (5 µm, 150 × 4.5 mm) with an isocratic mobile phase of buffer (pH 3) and methanol (40:60 v/v) at 1.0 mL min⁻¹ and 215 nm detection.
Method greenness was quantitatively evaluated using the Analytical GREEnness (AGREE) metric and the Green Analytical Procedure Index (GAPI).
Linearity was confirmed across concentration ranges of 2.5–7.5 µg mL⁻¹ for dapagliflozin, 1.25–3.75 µg mL⁻¹ for linagliptin, and 125–375 µg mL⁻¹ for metformin.
Precision achieved percent relative standard deviation (% RSD) values under 2%, alongside acceptable recovery percentages meeting validation criteria.

Cite This Study

Rathod et al. (2025) studied this question.

synapsesocial.com/papers/6a75fae7f1e99ff85ab228e1 https://doi.org/10.53879/id.62.09.15501

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September 28, 2025INDIAN DRUGS

Development and Validation of Rp-HPLC for Simultaneous Quantitation of Linagliptin, Dapagliflozin Propanediol Monohydrate and Metformin Hydrochloride: Greenness Evaluation by Agree and Gapi Tools

View Full Paper

Ask AI

Bookmark

Authors

SRSapna M. Rathod SPSayema Pothigara NPNisarg C. Patel

Discussion

Member takes

Overview

Method validation study demonstrates precise simultaneous RP-HPLC quantitation of linagliptin, dapagliflozin, and metformin, indicating its utility for eco-friendly pharmaceutical quality control.

Key Points

To develop and validate a precise, cost-effective, and environmentally sustainable RP-HPLC method for the simultaneous quantification of dapagliflozin, linagliptin, and metformin.
Chromatographic separation was achieved on an Agilent Eclipse XBD C18 column (5 µm, 150 × 4.5 mm) with an isocratic mobile phase of buffer (pH 3) and methanol (40:60 v/v) at 1.0 mL min⁻¹ and 215 nm detection.
Method greenness was quantitatively evaluated using the Analytical GREEnness (AGREE) metric and the Green Analytical Procedure Index (GAPI).
Linearity was confirmed across concentration ranges of 2.5–7.5 µg mL⁻¹ for dapagliflozin, 1.25–3.75 µg mL⁻¹ for linagliptin, and 125–375 µg mL⁻¹ for metformin.
Precision achieved percent relative standard deviation (% RSD) values under 2%, alongside acceptable recovery percentages meeting validation criteria.

Cite This Study

Rathod et al. (2025) studied this question.

synapsesocial.com/papers/6a75fae7f1e99ff85ab228e1 https://doi.org/10.53879/id.62.09.15501

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