Key Points
- To determine whether interferon-stimulated gene factors interact with interferon-stimulated response elements in vivo and to characterize their transcription dynamics across cell types.
- Mapped constitutive and interferon-inducible DNase hypersensitive sites within interferon-stimulated gene promoters in vivo.
- Assessed in vitro binding profiles of constitutive and inducible regulatory proteins and tracked corresponding gene transcription kinetics in multiple cell types.
- In vivo DNase hypersensitive sites located precisely near promoter sequences that bind constitutive or interferon-inducible proteins in vitro.
- Induction of hypersensitive sites and regulatory protein binding occurred either transiently or in a sustained manner depending on cell type.
- Interferon-stimulated gene transcription closely mirrored chromatin site induction, displaying either transient or maintained expression in parallel.
Structured PICO
PPopulationIn vivo models (specific cell types not detailed in abstract)
IInterventionInterferon stimulation
CComparatorConstitutive state (unstimulated)
OOutcomeInteraction between Interferon-Stimulated Gene Factors and the Interferon-Stimulated Response Elementsurrogate
The study provides in vivo evidence of the interaction between interferon-stimulated gene factors and response elements, showing that gene transcription parallels the transient or maintained nature of induced sites and proteins.