Why the study?
Does SPCE improve specificity and sensitivity compared to LPBE for detecting FMDV antibodies in cattle?
Does SPCE improve specificity and sensitivity compared to LPBE for detecting FMDV antibodies in cattle?
The SPCE method is more reliable, specific, and sensitive than LPBE for detecting FMDV antibodies in cattle, making it more suitable for evaluating vaccination programs.
SPCE may improve FMDV serosurveillance in vaccinated cattle; leaves open optimal assay choice pending validation studies.
The study was conducted using two ELISA methods - the liquid phase blocking ELISA (LPBE) and solid phase competition ELISA (SPCE) for the detection of foot-and-mouth disease virus (FMDV) serotype A- and O-specific antibodies of different cattle breeds in Turkey. These methods were compared in 426 cattle previously vaccinated with oil-adjuvanted bivalent vaccine as well as in sera from 40 cattle with no history of foot-and-mouth disease infection or vaccination. The results were found that SPCE had a better specificity (serotype A; 100% and serotype O; 97.50%) than LPBE (serotype A 95.00% and serotype O 92.50%). Sensitivity of SPCE had also better values (serotype A; 99.30% and serotype O; 98.59%) than LPBE (serotype A; 97.89% and serotype O; 96.48%). The results of the present study showed that the SPCE method is more reliable than LPBE.
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İnce et al. (2018) studied this question.
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