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In vitro study demonstrates growth of anaerobic fungi isolated from sheep and cattle digesta without rumen fluid, indicating viable synthetic cultivation.
Summary: Anaerobic fungi were isolated from rumen digesta of sheep and cattle and were purified using a plate culture technique. The isolates were successfully cultured on a semi-defined medium which lacked rumen fluid, and on a defined medium.
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Lowe et al. (1985) studied this question.
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