What question did this study set out to answer?

To develop and evaluate a sensitive, specific HPLC-MS/MS method capable of quantifying low concentrations of the algal hepatotoxin cylindrospermopsin in environmental water bodies.

February 1, 1999Environmental Toxicology

Use of HPLC-MS/MS to monitor cylindrospermopsin, a blue-green algal toxin, for public health purposes

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Authors

GEG. EagleshamQueensland Health RNRoss NorrisUNSW Sydney GSG. R. ShawGriffith University

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Implication

Laboratory assay study reveals reliable detection of trace cylindrospermopsin in water samples, highlighting an effective tool for public health monitoring during harmful algal blooms.

Key Points

To develop and evaluate a sensitive, specific HPLC-MS/MS method capable of quantifying low concentrations of the algal hepatotoxin cylindrospermopsin in environmental water bodies.
Lysed intact algal cells via freeze-thaw treatment and filtered samples through a 0.45 μm filter prior to a 110 μL injection.
Separated analytes on an Altima C18 column (250 × 4.6 mm, 5 μm) at 40°C using a 1–60% methanol gradient buffered with 5 mM ammonium acetate.
Monitored the mass-to-charge precursor-to-product ion transition from 416 m/z to 194 m/z using tandem mass spectrometry.
The assay demonstrated linear response across 1–600 μg/L [peak area = 304 × CYN (μg/L) − 569; r² = 1.000 (n = 7)].
Total coefficients of variation using a single-point standard curve were 26.4%, 10.5%, 12.6%, and 10.7% at concentrations of 0.78, 5.2, 104, and 1040 μg/L, respectively.

Cite This Study

Eaglesham et al. (1999) studied this question.

synapsesocial.com/papers/6a760b5ff1e99ff85ab22faf https://doi.org/10.1002/(sici)1522-7278(199902)14:1<151::aid-tox19>3.0.co;2-d

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Implication

Laboratory assay study reveals reliable detection of trace cylindrospermopsin in water samples, highlighting an effective tool for public health monitoring during harmful algal blooms.

Use of HPLC-MS/MS to monitor cylindrospermopsin, a blue-green algal toxin, for public health purposes

Discussion

Member takes

Implication

Key Points

Cite This Study

Use of HPLC-MS/MS to monitor cylindrospermopsin, a blue-green algal toxin, for public health purposes

Discussion

Member takes

Implication

Key Points

Cite This Study

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