Effects of the fat loading on oxidative stress in atherosclerosis

Effects of the fat loading on oxidative stress in atherosclerosisTo the Editor Atherosclerosis refers to the thickening and hardening of the arterial wall, loss of elasticity and narrowing of lumen. 1 It has been suggested that postprandial hyperlipidemia is an important factor that leads to atherosclerosis, and an independent risk factor for coronary heart disease, which can better predict coronary heart disease. 2 Hyperlipidemia can enhance the activity of various cells in the arterial wall, and promote these to continuously generate and release ROS, which in turn would continue to oxidize the lipid components and enhance the lipid peroxidation. 3,4Therefore, we conducted this study to investigate the effects of fat loading on oxidative stress during the course of atherosclerosis.In the present study, 10 New Zealand male white rabbits were fed with high-fat diet for 12 weeks at the beginning, and at the 4th, 8th and 12th week after fat loading, We detected The plasma level of superoxide dismutase (SOD), malondialdehyde (MDA), myeloperoxidase (MPO) and glutathione peroxidase (GSH-px) levels, ultrasound was used to evaluate the status of the atherosclerosis in the abdominal aorta.Then, these animals were euthenized and the atherosclerosis was observed by H&E staining after 12 weeks.We revealed that after being continuously fed with a high-fat diet, the level of SOD decreased, while the level of MDA increased, in the 4 th and 8 th week.Furthermore, the level of MPO increased in the 8th and 12 th week, while the level of GSH-px continuously decreased in the 4 th , 8 th and 12 th week.After fat loading, the level of SOD was the lowest at 4 h postprandial, at the beginning and at 4 th week.Furthermore, the level of MDA was the highest at 2 h postprandial, at each observation point.Moreover, the level of MPO continuously decreased at the 4 th and 8 th week, while the level of GSH-px gradually decreased (Fig. 1).In conclusion, regardless of whether it is a long-term high-fat