High-resolution tracking of microtubule motility driven by a single kinesin motor.

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Structured PICO

Population

Single kinesin motor and microtubules

Intervention

High-resolution tracking using a microscope capable of tracking movement with 1-nm resolution at a bandwidth of 200 Hz

Outcome

Microtubule movement and translocation distance per ATP hydrolysis cycle

Kinesin likely moves forward by the distance of only a single tubulin subunit under zero load conditions during its ATP hydrolysis cycle.

Brownian forces acting on elastic elements within the kinesin motor precluded detection of steps that were < 12 nm

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Supports single-tubulin stepping under zero load; leaves open load- or context-dependent multi-step behavior in cells.

Malik et al. (1994) studied this question.

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