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Investigate the molecular mechanisms by which alpha1,6-fucosyltransferase deficiency and loss of core fucosylation disrupt epidermal growth factor receptor-mediated cellular signaling.

November 29, 2005Journal of Biological ChemistryOpen Access

Core Fucosylation Regulates Epidermal Growth Factor Receptor-mediated Intracellular Signaling

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Investigate the molecular mechanisms by which alpha1,6-fucosyltransferase deficiency and loss of core fucosylation disrupt epidermal growth factor receptor-mediated cellular signaling.
Derived embryonic fibroblasts from wild-type (Fut8+/+) and Fut8-knockout (Fut8-/-) mice.
Evaluated epidermal growth factor (EGF) receptor phosphorylation, tyrosine phosphatase activity, and downstream activation of JNK and ERK signaling pathways.
Assessed cell-surface receptor expression and EGF ligand binding, followed by rescue experiments via re-introduction of the Fut8 gene into Fut8-/- cells.
EGF-induced phosphorylation of EGFR was substantially blocked in Fut8-/- fibroblasts compared to Fut8+/+ cells, with receptor phosphorylation completely restored upon Fut8 gene re-introduction.
Downstream activation of JNK and ERK signaling pathways was significantly suppressed in Fut8-/- cells despite no differences in tyrosine phosphatase activity or total surface EGFR expression.
Core fucosylation of N-glycans was specifically required for the binding of EGF to its receptor.

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Implication

Experimental study demonstrates that loss of core fucosylation impairs epidermal growth factor receptor signaling in murine fibroblasts, indicating its necessity for normal growth.

Cite This Study

Wang et al. (2005) studied this question.

synapsesocial.com/papers/6a7620b18802f13c0e613f49 https://doi.org/10.1074/jbc.m510893200

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Implication

Experimental study demonstrates that loss of core fucosylation impairs epidermal growth factor receptor signaling in murine fibroblasts, indicating its necessity for normal growth.

Core Fucosylation Regulates Epidermal Growth Factor Receptor-mediated Intracellular Signaling

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Discussion

Implication

Cite This Study

Core Fucosylation Regulates Epidermal Growth Factor Receptor-mediated Intracellular Signaling

Key Points

Discussion

Implication

Cite This Study

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