The treatment of different DNAs with dimethylsulfate leads to the methylation of guanine and adenine residues. The amount of each methylated base depends on the base composition of the DNA and on the total yield of the reaction. Light scattering measurements show that there are no doublestrand breaks. Thermal denaturation and luminescence at room and low temperature indicate a stable secondary structure without local denaturation. The flexibility of methylated DNA is larger than that of unmethylated DNA as shown by the study of the intrinsic viscosity. Spermine decreases the thermal stability of methylated DNA. The association constants and the number of binding sites are changed by methylation as determined by equilibrium dialysis. The study of the interactions between poly-l-lysine and methylated DNA shows that methylated bases interfere with the selectivity of poly-l-lysine for A + T rich DNA. Some results on the transcription of methylated DNA by RNA polymerase are reported.
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Ramstein et al. (1971) studied this question.
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