The rhamnolipids produced by Pseudomonas aeruginosa strains are often a mixture of several homologues. Up to seven (R 2 C 10 C 10 + R 1 C 10 C 10 + R 2 C 10 C 12 + R 1 C 10 C 12 + R 1 C 12:1 C 10 + R 1 C 12:2 + R 1 C 8:2 ) have been identified in cultures of P. aeruginosa AT10 from soybean oil refinery wastes. This study deals with the production, purification, and identification of rhamnolipids in the mixture (M 7 ), as well as their physical and chemical characterization and the evaluation of their antimicrobial properties. The surface tension decreases to 26.8 mN/m and the critical micelle concentration value to 1.2 × 10 2 mg/L. These molecules show excellent antifungal properties against Aspergillus niger and Gliocadium virens (16 μg/mL) and C. globosum, P. crysogenum, and A. pullulans (32 μg/mL), whereas the growth of the phytopathogenic fungi B. Cinerea and R. solani was inhibited at 18 μg/mL.
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Ábalos et al. (2001) studied this question.
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