What question did this study set out to answer?

To determine whether Bacillus thuringiensis delta-endotoxin inhibits amino acid transport across the midgut brush border membrane of Pieris brassicae larvae by inactivating symporters or altering membrane permeability.

August 18, 1986FEBS LettersOpen Access

Bacillus thuringiensis toxin inhibits K+‐gradient‐dependent amino acid transport across the brush border membrane of Pieris brassicae midgut cells

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Authors

VSV.F. SacchiBrigham and Women's Hospital PPPaolo ParentiUniversity of Milano-Bicocca GHGiorgio M. HanozetUniversity of Milano-Bicocca

Discussion

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Overview

In vitro study reveals that Bacillus thuringiensis toxin inhibits amino acid uptake in caterpillar midgut cells, indicating selective disruption of potassium permeability.

Key Points

To determine whether Bacillus thuringiensis delta-endotoxin inhibits amino acid transport across the midgut brush border membrane of Pieris brassicae larvae by inactivating symporters or altering membrane permeability.
Prepared brush border membrane vesicles from the midguts of Pieris brassicae larvae and mammalian small intestinal tissue.
Treated vesicles with insecticidal Bacillus thuringiensis delta-endotoxin at concentrations reflecting normal in vivo effective doses.
Measured amino acid uptake and vesicular membrane permeability to potassium (K+), sodium (Na+), and hydrogen (H+) ions.
Delta-endotoxin inhibited amino acid uptake in Pieris brassicae midgut vesicles by increasing membrane permeability to K+ rather than interacting directly with the K+-amino acid symport.
Toxin exposure did not alter membrane permeability to Na+ or H+ in larval midgut vesicles.
Toxin exposure caused no increase in K+ permeability in brush border membrane vesicles prepared from mammalian small intestine.

Cite This Study

Sacchi et al. (1986) studied this question.

synapsesocial.com/papers/6a767a8053fd60d5b195ca93 https://doi.org/10.1016/0014-5793(86)80814-6

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August 18, 1986FEBS LettersOpen Access

Bacillus thuringiensis toxin inhibits K+‐gradient‐dependent amino acid transport across the brush border membrane of Pieris brassicae midgut cells

View Full Paper

Ask AI

Bookmark

Authors

VSV.F. SacchiBrigham and Women's Hospital PPPaolo ParentiUniversity of Milano-Bicocca GHGiorgio M. HanozetUniversity of Milano-Bicocca

Discussion

Member takes

Overview

In vitro study reveals that Bacillus thuringiensis toxin inhibits amino acid uptake in caterpillar midgut cells, indicating selective disruption of potassium permeability.

Key Points

To determine whether Bacillus thuringiensis delta-endotoxin inhibits amino acid transport across the midgut brush border membrane of Pieris brassicae larvae by inactivating symporters or altering membrane permeability.
Prepared brush border membrane vesicles from the midguts of Pieris brassicae larvae and mammalian small intestinal tissue.
Treated vesicles with insecticidal Bacillus thuringiensis delta-endotoxin at concentrations reflecting normal in vivo effective doses.
Measured amino acid uptake and vesicular membrane permeability to potassium (K+), sodium (Na+), and hydrogen (H+) ions.
Delta-endotoxin inhibited amino acid uptake in Pieris brassicae midgut vesicles by increasing membrane permeability to K+ rather than interacting directly with the K+-amino acid symport.
Toxin exposure did not alter membrane permeability to Na+ or H+ in larval midgut vesicles.
Toxin exposure caused no increase in K+ permeability in brush border membrane vesicles prepared from mammalian small intestine.

Cite This Study

Sacchi et al. (1986) studied this question.

synapsesocial.com/papers/6a767a8053fd60d5b195ca93 https://doi.org/10.1016/0014-5793(86)80814-6

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