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SUMMARY As the osmolarity of the medium decreases, ‘platelet factor‐3’ (PF‐3) availability increases in erythrocyte as well as platelet suspensions. Associated with this is a parallel increase in PF‐3 activity of the cell‐free supernatant. Addition of glycerol to platelet or erythrocyte suspensions enhances PF‐3 availability of the cell suspensions but not the cell‐free supernatants. It is proposed that glycerol influx alters the orientation or binding of membrane thromboplastic phospholipids, thereby facilitating their reactivity in the PF‐3 assay.
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Phin Cohen (1967) studied this question. Decreased medium osmolarity and addition of glycerol was evaluated on Platelet factor-3 (PF-3) availability and activity in erythrocyte and platelet suspensions and cell-free supernatants.
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