cause of the illness. The PCR for amplification of a 470-bp fragment of citrate synthase gene was performed according to a previously published protocol (5). DNA was extracted with QIAamp DNA Mini Kit (QIAGEN, Hilden, Germany) from whole blood and the crust of the eschar collected on day 5 of illness. In addition, serum samples were tested by indirect immunofluorescent assay for specific IgG and IgM against Francisella tularensis and Rickettsia spp. (spotted fever and typhus group) 5 days and 10 weeks after onset of fever.
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Bartolleti et al. (2016) studied this question.
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