August 1, 1984Biochemistry
Characterization of the properties of ethenoadenosine nucleotides bound or trapped at the active site of myosin subfragment 1
Population
Myosin subfragment 1 (SF1)
Comparison
Thiol cross-linking agents of different spanning… vs Reversibly bound epsilon ADP and free epsilon ADP
Design
Preclinical
Authors
Discussion
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Implication
Should not change cardiovascular practice; hypothesis-generating for myosin-targeted drug design.
Structured PICO
P
Population
Myosin subfragment 1 (SF1)
I
Intervention
Thiol cross-linking agents of different spanning lengths (2-14 A) and p-phenylenedimaleimide
C
Comparator
Reversibly bound epsilon ADP and free epsilon ADP
O
Outcome
Fluorescent properties, polarization, off-rate, and solute quencher accessibility of trapped epsilon ADPsurrogate
Trapping of ADP at the myosin active site by thiol cross-linking agents likely occurs via indirect stabilization of a conformation favoring bound nucleotides rather than direct steric hindrance.
Cite This Study
Perkins et al. (1984) studied this question.