What question did this study set out to answer?

To characterize the dynamics and mechanisms of intracellular calcium mobilization stimulated by extracellular ATP in Ehrlich ascites tumor cells.

September 1, 1985Journal of Biological ChemistryOpen Access

Intracellular Ca2+ mobilization activated by extracellular ATP in Ehrlich ascites tumor cells.

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Authors

GDGeorge DubyakCase Western Reserve University MYM. B. De YoungUniversity of Pennsylvania

Discussion

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Implication

In vitro study demonstrates dose-dependent calcium mobilization induced by extracellular ATP in tumor cells, suggesting hormone-like signaling activity.

Key Points

To characterize the dynamics and mechanisms of intracellular calcium mobilization stimulated by extracellular ATP in Ehrlich ascites tumor cells.
Exposed Ehrlich ascites tumor cells to 0.4–25 µM extracellular ATP in the presence of 1 mM extracellular Ca2+.
Evaluated Ca2+ transient kinetics, reversibility via enzymatic degradation (ecto-ATPase or hexokinase), and responsiveness to alternative nucleotide triphosphates (ITP, UTP, GTP) and non-hydrolyzable ATP analogs.
Extracellular ATP induced a triphasic Ca2+ transient characterized by an initial rapid release from non-mitochondrial internal stores (2–3 s), a secondary influx across the plasma membrane (60–90 s), and recovery to baseline (4–5 min).
Nucleotide triphosphates ITP, UTP, and GTP produced identical Ca2+ transients without altering general membrane permeability, whereas non-hydrolyzable ATP analogs exhibited no activity or antagonism.

Cite This Study

Dubyak et al. (1985) studied this question.

synapsesocial.com/papers/6a76af9ef6749a0058ec2a0c https://doi.org/10.1016/s0021-9258(19)85134-7

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September 1, 1985Journal of Biological ChemistryOpen Access

Intracellular Ca2+ mobilization activated by extracellular ATP in Ehrlich ascites tumor cells.

View Full Paper

Ask AI

Bookmark

Authors

GDGeorge DubyakCase Western Reserve University MYM. B. De YoungUniversity of Pennsylvania

Discussion

Member takes

Implication

In vitro study demonstrates dose-dependent calcium mobilization induced by extracellular ATP in tumor cells, suggesting hormone-like signaling activity.

Key Points

To characterize the dynamics and mechanisms of intracellular calcium mobilization stimulated by extracellular ATP in Ehrlich ascites tumor cells.
Exposed Ehrlich ascites tumor cells to 0.4–25 µM extracellular ATP in the presence of 1 mM extracellular Ca2+.
Evaluated Ca2+ transient kinetics, reversibility via enzymatic degradation (ecto-ATPase or hexokinase), and responsiveness to alternative nucleotide triphosphates (ITP, UTP, GTP) and non-hydrolyzable ATP analogs.
Extracellular ATP induced a triphasic Ca2+ transient characterized by an initial rapid release from non-mitochondrial internal stores (2–3 s), a secondary influx across the plasma membrane (60–90 s), and recovery to baseline (4–5 min).
Nucleotide triphosphates ITP, UTP, and GTP produced identical Ca2+ transients without altering general membrane permeability, whereas non-hydrolyzable ATP analogs exhibited no activity or antagonism.

Cite This Study

Dubyak et al. (1985) studied this question.

synapsesocial.com/papers/6a76af9ef6749a0058ec2a0c https://doi.org/10.1016/s0021-9258(19)85134-7

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