The aim of this study was to investigate the effects of paeoniflorin (PF) and paeonol (PN), the main active compounds of the Paeonia albiflora Pallas, on myocardial ischemia and reperfusion (I/R)-induced injury in Sprague-Dawley rats in vivo . Under anesthesia, the rats were subjected to 25 min of ischemia by ligation of the left anterior descending coronary artery (LAD) followed by 6 h (Western blot analysis) or 24 h (hemodynamics and infarct size) of reperfusion. When the infarct size was measured as the percentage of the area at risk, both PF (25.0 % ± 7.0 %) and PN (24.1 % ± 5.5 %) significantly (P < 0.05) reduced it compared to I/R control (54.8 % ± 2.6 %). Administration of 10 mg/kg PF or PN 1 h prior to I/R injury also resulted in a significant improvement of the hemodynamic parameters. Furthermore, both PF and PN decreased the caspase-3 and Bax expressions but up-regulated Bcl-2 in the left ventricles. The results show that both PF and PN reduced myocardial damage in rat through protection from apoptosis, suggesting that Paeonia albiflora Pallas might be useful in treating myocardial infarction. Abbreviations AAR:area at risk ANOVA:analysis of variance DBP:diastolic blood pressure HR:heart rate ICAM:intercellular adhesion molecule JNK:c-Jun N -terminal kinase LAD:left anterior descending coronary artery LV:left ventricle LVSP:left ventricle systolic pressure LVDP:left ventricle developed pressure LVEDP:left ventricle end-diastolic pressure MAP:mean arterial pressure MAPK:mitogen-activated protein kinase NF-κB:nuclear factor kappa B SBP:systolic blood pressure SDS:sodium dodecyl sulfate PF:paeoniflorin PN:paeonol PVDF:polyvinylidene difluoride TBS-T:Tris-buffered saline/Tween 20 TNF:tumor necrosis factor VCAM:vascular cell adhesion molecule
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Nizamutdinova et al. (2008) studied this question.