Rats were made polyuric by the addition of lithium chloride to the food. After three weeks, when the polyuria was constant, the renal adenyl cyclase activity was determined and compared with the activity in kidneys of control rats not given lithium. Adenyl cyclase activities were determined as the amount of cyclic AMP‐ 14 C formed from ATP‐ 14 C; cyclic AMP was isolated by the method of Krishna & Birnbaumer (1970). Polyuric rats and control rats did not differ as regards the unstimulated and the fluoride‐stimulated adenyl cyclase activities, but the polyuric rats showed a lower vasopressin‐induced increase (39 per cent) than did the control rats (69 per cent). The lithium concentration in the incubate was below the limit of detection. Our results indicate that inhibition of the vasopressin‐stimulated adenyl cyclase in the kidney is the cause of the vasopressin‐resistant polyuria produced by lithium.
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Geisler et al. (1972) studied this question.
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