Towards point-of-care tests for protein detection at the attomolar level via disposable pollen-based nanoplasmonic probes grafted with polymer-based receptors

A point-of-care test (POCT) based on low-cost and highly sensitive disposable chips was designed for the sensitive and selective detection of proteins. In particular, a pollen-based plasmonic nanostructured probe coupled, for the first time, with biomimetic receptors custom-designed as molecularly imprinted nanoparticles (MIP-NPs) for protein recognition, was developed and interrogated by an extrinsic optical fiber (OF)-based scheme. To this purpose, bovine serum albumin (BSA) was chosen in a proof-of-concept frame as an example of a protein.