What question did this study set out to answer?

To investigate whether genetic mutations or altered expression of the tumour suppressor PTEN correlate with the invasive, cartilage-destructive phenotype of synovial fibroblasts in rheumatoid arthritis.

December 6, 1999Arthritis Research & TherapyOpen Access

Activation of synovial fibroblasts in rheumatoid arthritis: lack of expression of the tumour suppressor PTEN at sites of invasive growth and destruction

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Authors

TPThomas PapSemmelweis University JFJuliane K. FranzDeutsches Herzzentrum der Charité KHKlaus M. HummelUniversity Hospital Carl Gustav Carus

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Implication

Experimental study reveals lack of PTEN expression in invasive rheumatoid arthritis synovial fibroblasts, suggesting endogenous downregulation contributes to joint destruction.

Key Points

To investigate whether genetic mutations or altered expression of the tumour suppressor PTEN correlate with the invasive, cartilage-destructive phenotype of synovial fibroblasts in rheumatoid arthritis.
Extracted RNA from synovial fibroblasts of 7 rheumatoid arthritis patients and 2 nonarthritic controls, followed by PCR amplification and automated sequencing of a 1,063 base pair PTEN coding fragment.
Conducted in situ hybridization for PTEN and MMP-2 mRNA on intact synovial tissues, cultured synovial fibroblasts across six passages, and human cartilage co-implants in a SCID mouse model.
Sequencing revealed 100% homology to the wild-type PTEN sequence across all 9 cell cultures, indicating an absence of structural gene mutations.
In situ hybridization demonstrated negligible PTEN mRNA in the destructive synovial lining and cartilage-invading cells in SCID mice, despite abundant PTEN expression in synovial sublining layers and normal tissue.
PTEN mRNA was expressed in only approximately 40% of cultured rheumatoid arthritis synovial fibroblasts, remaining constant across six passages, while MMP-2 was constitutively expressed in nearly all cells.

Cite This Study

Pap et al. (1999) studied this question.

synapsesocial.com/papers/6a7715adee59ab58d3f6c796 https://doi.org/10.1186/ar69

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Implication

Experimental study reveals lack of PTEN expression in invasive rheumatoid arthritis synovial fibroblasts, suggesting endogenous downregulation contributes to joint destruction.

Activation of synovial fibroblasts in rheumatoid arthritis: lack of expression of the tumour suppressor PTEN at sites of invasive growth and destruction

Discussion

Member takes

Implication

Key Points

Cite This Study

Activation of synovial fibroblasts in rheumatoid arthritis: lack of expression of the tumour suppressor PTEN at sites of invasive growth and destruction

Discussion

Member takes

Implication

Key Points

Cite This Study

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