What question did this study set out to answer?

To establish and evaluate a simple, reproducible method for purifying plasma membranes from fresh ejaculated boar spermatozoa.

February 1, 1995Preparative Biochemistry

A Simple Technique for the Purification of Plasma Membranes from Ejaculated Boar Spermatozoa

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Authors

GAGary C. AlthouseUniversity of Pennsylvania KBKerry A. BrunsSouthwestern University LELawrence E. EvansAlpine Immune Sciences (United States)

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Implication

Laboratory study demonstrates efficient isolation of intact plasma membranes in boar spermatozoa, highlighting a simple protocol for reproductive membrane analysis.

Key Points

To establish and evaluate a simple, reproducible method for purifying plasma membranes from fresh ejaculated boar spermatozoa.
Separated spermatozoa (1 x 10^8 cells/ml) from fresh boar ejaculates using a 1.0 M sucrose density gradient.
Subjected cells to gas cavitation at 650 psi for 10 minutes, followed by a 4-step centrifugation procedure.
Evaluated isolate purity using differential interference contrast, transmission electron microscopy, and enzyme markers for plasma, acrosomal, and mitochondrial membranes.
Gas cavitation selectively removed plasma membranes primarily from the periacrosomal region while leaving acrosomes visibly intact.
Transmission electron microscopy revealed a homogenous yield of 100-200 micron unilamellar vesicles.
Assays of specific enzyme markers confirmed the visual purity of the isolated plasma membrane fraction with negligible organelle contamination.

Cite This Study

Althouse et al. (1995) studied this question.

synapsesocial.com/papers/6a7716f4e29fd788c9f70aa9 https://doi.org/10.1080/10826069508010108

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Implication

Laboratory study demonstrates efficient isolation of intact plasma membranes in boar spermatozoa, highlighting a simple protocol for reproductive membrane analysis.

A Simple Technique for the Purification of Plasma Membranes from Ejaculated Boar Spermatozoa

Discussion

Member takes

Implication

Key Points

Cite This Study

A Simple Technique for the Purification of Plasma Membranes from Ejaculated Boar Spermatozoa

Discussion

Member takes

Implication

Key Points

Cite This Study

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